How to get peptides tested, step by step
Short answer: buy or set aside a sealed vial, choose an independent analytical lab that runs LC-MS and HPLC on peptides, order identity, purity and net content testing, send the vial with its lot number recorded, then compare the lab's report with the supplier's COA for the same lot.
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1. Pick the sample
- Use a sealed, unopened vial in its original packaging.
- Write down the lot number and photograph the label before you send it.
- For the strongest check, test a vial bought the way any customer would buy it, not one the supplier picked for you.
2. Choose a lab
Look for an independent lab, with no ties to the supplier, that tests peptides by LC-MS and HPLC and lets you verify its reports. Check any accreditation claim with the body that issued it. ISO/IEC 17025 is the one that covers testing competence. The full criteria are in how to judge a peptide testing lab.
3. Order the right tests
| Test | Answers | Usual method |
|---|---|---|
| Identity | Is it the compound on the label? | LC-MS or LC-MS/MS |
| Purity | How much of the detected material is that compound? | HPLC with UV detection |
| Net content | How many mg of peptide are actually in the vial, against the label? | HPLC quantitation |
Purity alone isn't enough. A vial can be pure and still be the wrong compound, or contain less than the label says. See what a purity percentage leaves out.
4. Send it
- Fill in the lab's submission form: compound name, label amount, lot number, and the expected molecular mass if you have it.
- Pack the vial so it can't break or leak, and follow the lab's shipping instructions.
- Keep the tracking number and your photos with your copy of the form.
5. Read the report
Check the lab's report against the supplier's COA for the same lot: identity, purity, net content, lot number and dates. A different lab using different settings can report a somewhat different purity figure, so judge any gap against the supplier's stated pass mark and look at the chromatogram. Then confirm the report with the lab's own lookup. Our COA reading guide goes line by line.
Where can I get my peptides tested?
Several independent labs accept peptide samples from the public and publish COA lookup pages. Before you rely on one, run it through the five checks in how to judge a lab, especially accreditation and public report verification.
Frequently asked questions
Where can I get my peptides tested?
At an independent analytical lab that tests peptides by LC-MS and HPLC, accepts samples from the public and lets you verify its reports online.
Which tests should I order?
Identity by LC-MS, purity by HPLC with the chromatogram, and net content. Purity alone can't tell you whether the compound or the amount is right.
Can I test peptides at home?
Not in the way a COA does. Purity and identity require HPLC and mass spectrometry, which are laboratory instruments.
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